Composite

Part:BBa_K3425053

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-18)


Qβ3' recognition sequence of Qβ replicase with terminator

BBa_K3425053 is a construct containing the Qβ3' recognition sequence of Qβ replicase, BBa_K3425039, fused with the standard terminator BBa_B0015.

QB recognition site
Refereces
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/bacteriophage
//chassis/prokaryote/ecoli
//classic/rna/uncategorized
//direction/reverse
Parameters
chassisE. coli
device_typeRNA functional element
functionRNA amplification